The anaerobic gut fungi (AGF; phylum Neocallimastigomycota) reside in the alimentary tracts of herbivores. Multiple novel, yet-uncultured AGF taxa have just lately been recognized in culture-independent range surveys. Here, we report on the isolation and characterization of the first consultant of the RH5 lineage from faecal samples of a wild blackbuck (Indian Antelope, Antilope cervicapra) from Sutton County, Texas, USA. The isolates displayed medium sized (2-Four mm) compact round colonies on agar roll tubes and skinny free biofilm-like progress in liquid medium.
Microscopic examination revealed monoflagellated zoospores and polycentric thalli with extremely branched nucleated filamentous rhizomycelium, a progress sample encountered in a minority of described AGF genera to date. The obtained isolates are characterised by formation of spherical vesicles at the hyphal ideas from which a number of sporangia fashioned both straight on the spherical vesicles or at the finish of sporangiophores.
Phylogenetic evaluation utilizing the D1/D2 areas of the massive ribosomal subunit (D1/D2 LSU) and the ribosomal inner transcribed spacer 1 (ITS1) revealed sequence similarities of 93.5 and 81.3%, respectively, to the closest cultured kinfolk (Orpinomyces joyonii pressure D3A (D1/D2 LSU) and Joblinomyces apicalis pressure GFH681 (ITS1).
Substrate utilization experiments utilizing the kind pressure (BB-3T) demonstrated progress capabilities on a wide selection of mono-, oligo- and polysaccharides, together with glucose, xylose, mannose, fructose, cellobiose, sucrose, maltose, trehalose, lactose, cellulose, xylan, starch and raffinose. We suggest accommodating these novel isolates in a new genus and species, for which the identify Paucimyces polynucleatus gen. nov., sp. nov. is proposed.
Description of the Marine Predator Sericomyxa perlucida gen. et sp. nov., a Cultivated Representative of the Deepest Branching Lineage of Vampyrellid Amoebae (Vampyrellida, Rhizaria)
The vampyrellids (Vampyrellida, Rhizaria) are an order of bare amoebae of appreciable genetic range. Three households have been nicely outlined (Vampyrellidae, Leptophryidae, Placopodidae), however most vampyrellid lineages detected by environmental sequencing are poorly recognized or utterly uncharacterised. In the brackish sediment of Lake Bras D’Or, Nova Scotia, Canada, we found an amoeba with a vampyrellid-like life historical past that was morphologically dissimilar from recognized vampyrellid taxa.
We established a tradition of this amoeba, studied its feeding behaviour and prey vary specificity, and characterised it with molecular phylogenetic strategies and lightweight and electron microscopy. The amoeba was a generalist predator (i.e. eukaryotroph), devouring a vary of marine microalgae, with a robust affinity for some benthic diatoms and Chroomonas. Interestingly, the amoeba diverse its feeding technique relying on the prey species. Small diatoms had been engulfed complete, whereas bigger species had been ate up by extraction with an invading pseudopodium.

The SSU rRNA gene phylogenies robustly positioned the amoeba in the most basal, poorly described lineage (‘clade C’) of the Vampyrellida. Based on the phylogenetic place and the distinct morphology of the studied amoeba, we right here describe it as Sericomyxa perlucida gen. et sp. nov., and set up the new vampyrellid household Sericomyxidae for ‘clade C’.
Fensomea setacea, gen. & sp. nov. (Cladopyxidaceae, Dinophyceae), is neither gonyaulacoid nor peridinioid as inferred from morphological and molecular knowledge
Dinophyte evolution is actually inferred from the sample of thecal plates, and two totally different labelling techniques are used for the vital subgroups Gonyaulacales and Peridiniales. The partiform hypotheca of cladopyxidoid dinophytes matches into the morphological ideas of neither group, though they’re assigned to the Gonyaulacales. Here, we describe the thecate dinophyte Fensomea setacea, gen. & sp. nov., which has a cladopyxidoid tabulation.
The cells displayed a Kofoidean plate system APC, 3′, 4a, 7″, 7C, 6S, 6”’, 2””, and slender processes had been randomly distributed over the echinate or baculate floor. In addition, we obtained rRNA sequences of F. setacea, gen. & sp. nov., however dinophytes that exhibit a partiform hypotheca didn’t present a shut relationship to Gonyaulacales. Character evolution of thecate dinophytes could have progressed from the ancestral state of six postcingular plates, and two kind of symmetrically organized antapical plates, in direction of patterns of solely 5 postcingular plates (Peridiniales) or extra asymmetrical configurations (Gonyaulacales).
Based on our phylogenetic reconsiderations the contact between the posterior sulcal plate and the first postcingular plate, in addition to the contact between an antapical plate and the distalmost postcingular plate, don’t signify a uncommon, specialised gonyaulacoid plate configuration (i.e., the partiform hypotheca of cladopyxidoid dinophytes). Instead, these contacts correspond to the widespread and common configuration of peridinioid (and different) dinophytes.
Guiclupea superstes, gen. et sp. nov., the youngest ellimmichthyiform (clupeomorph) fish to this point from the Oligocene of South China
A brand new ellimmichthyiform, Guiclupea superstes, gen. et sp. nov., from the Oligocene Ningming Formation of Ningming Basin, Guangxi Zhuang Autonomous Region, South China is described herein. With comparatively massive physique dimension, parietals assembly at the midline, anterior ceratohyal with a beryciform foramen in the heart, a full predorsal scutes sequence of very excessive quantity and about equally-size scutes with radiating ridges on dorsal floor, first preural centrum unfused with first uroneural however fused with the parhypural, and first ural centrum of roughly the similar dimension as the preural centrum, Guiclupea superstes can’t be assigned to the order Clupeiformes.
The egg drop syndrome (76) PCR kit |
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PCR-V044-96D | Bioingentech | 100T | 686.4 EUR |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-100D | Bioingentech | 100T | 1039.2 EUR |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-150D | Bioingentech | 150T | 1177.2 EUR |
Egg Drop Syndrome One-Step PCR kit |
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Oneq-V026-50D | Bioingentech | 50T | 861.6 EUR |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-100D | Bioingentech | 100T | 860.4 EUR |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-150D | Bioingentech | 150T | 969.6 EUR |
The egg drop syndrome (76) RT PCR kit |
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RTq-V044-50D | Bioingentech | 50T | 717.6 EUR |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-100D | Bioingentech | 100T | 1039.2 EUR |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-150D | Bioingentech | 150T | 1177.2 EUR |
The egg drop syndrome (76) One-Step PCR kit |
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Oneq-V044-50D | Bioingentech | 50T | 861.6 EUR |
PCR Mix |
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L5051100 | Biochain | 2.5 ml | 67 EUR |
PRICE DROP |
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110-012 | GeneOn | 4 x 1000 µl | 159.6 EUR |
PRICE DROP |
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110-012L | GeneOn | 5x 4 x 1000 µl | 621.6 EUR |
PRICE DROP |
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110-012XL | GeneOn | 10x 4 x 1000 µl | 1126.8 EUR |
Ready? PCR Mix |
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M1127-1000 | Biovision | each | 614.4 EUR |
Ready? PCR Mix |
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M1127-200 | Biovision | each | 229.2 EUR |
Rigor? PCR Mix |
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M1132-200 | Biovision | each | 385.2 EUR |
Breeze? PCR Mix |
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M1134-200 | Biovision | each | 320.4 EUR |
Distant? PCR Mix |
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M1136-200 | Biovision | each | 352.8 EUR |
Advance? PCR Mix |
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M1139-200 | Biovision | each | 451.2 EUR |
Fire Start? PCR Mix |
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M1141-200 | Biovision | each | 451.2 EUR |
Ready? PCR Mix-Dye |
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M1128-1000 | Biovision | each | 614.4 EUR |
Ready? PCR Mix-Dye |
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M1128-200 | Biovision | each | 229.2 EUR |
Image Ready? PCR Mix |
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M1129-200 | Biovision | each | 352.8 EUR |
Rigor? PCR Mix-Dye |
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M1133-200 | Biovision | each | 385.2 EUR |
Whole Blood PCR Mix |
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M1143-200 | Biovision | each | 385.2 EUR |
Robust Ready? PCR Mix |
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M1130-1000 | Biovision | each | 738 EUR |
Robust Ready? PCR Mix |
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M1130-200 | Biovision | each | 255.6 EUR |
Breeze? PCR Mix-Dye |
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M1135-200 | Biovision | each | 320.4 EUR |
Distant? PCR Mix-Dye |
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M1137-200 | Biovision | each | 352.8 EUR |
Image Distant? PCR Mix |
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M1138-200 | Biovision | each | 385.2 EUR |
Advance? PCR Mix-Dye |
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M1140-200 | Biovision | each | 451.2 EUR |
PCR-EZ D-PCR MASTER MIX |
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BS294 | Bio Basic | 100RXN, 100prep | 112.2 EUR |
amfiSure PCR Master Mix |
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P0311-010 | GenDepot | 2x50 rxns | 151.2 EUR |
amfiSure PCR Master Mix |
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P0311-025 | GenDepot | 5X50 rxns | 198 EUR |
amfiSure PCR Master Mix |
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P0311-050 | GenDepot | 10X50 rxns | 262.8 EUR |
amfiSure PCR Master Mix |
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P0311-100 | GenDepot | 10x1ml | 343.2 EUR |
amfiSure PCR Master Mix |
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P0311-125 | GenDepot | 15X50 rxns | 333.6 EUR |
amfiSure PCR Master Mix |
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P0311-200 | GenDepot | 20x1ml | 562.8 EUR |
amfiSure PCR Master Mix |
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P0311-250 | GenDepot | 50x50 rxns | 902.4 EUR |
amfiSure PCR Master Mix |
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P0311-500 | GenDepot | 100x50 rxns | 1509.6 EUR |
amfiXpand PCR Master Mix |
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P0331-010 | GenDepot | 2X50 rxns | 181.2 EUR |
amfiXpand PCR Master Mix |
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P0331-025 | GenDepot | 5X50 rxns | 327.6 EUR |
amfiXpand PCR Master Mix |
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P0331-050 | GenDepot | 10X50 rxns | 537.6 EUR |
amfiXpand PCR Master Mix |
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P0331-250 | GenDepot | 50x50 rxns | 2247.6 EUR |
Taq 2x PCR Mix 100 rxn |
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BA01501 | Bioatlas | 100rxn | 112.8 EUR |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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MyTaq Blood PCR Mix, 2x |
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BIO-25053/S | Bioline | Sample | Ask for price |
MyTaq Blood PCR Mix, 2x |
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BIO-25054 | Bioline | 250 Reactions | Ask for price |
HotTaq 2x PCR Mix 100 rxn |
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BA01503 | Bioatlas | 100rxn | 144 EUR |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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RedTaq 2x PCR Mix 100 rxn |
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BA01507 | Bioatlas | 100rxn | 175.2 EUR |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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Fire Start? PCR Mix-Dye |
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M1142-200 | Biovision | each | 451.2 EUR |
HiScript-TS 2 × PCR Mix |
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RA103-01 | Vazyme | 40 rxns (25 μl/rxn) | 69.6 EUR |
HiScript-TS 2 × PCR Mix |
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RA103-02 | Vazyme | 200 rxns (25 μl/rxn) | 275.2 EUR |
Funnel Pyrex Cyl Drop Gr.500ml |
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FUN4024 | Scientific Laboratory Supplies | EACH | 176.4 EUR |
Robust Ready? PCR Mix-Dye |
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M1131-1000 | Biovision | each | 738 EUR |
Robust Ready? PCR Mix-Dye |
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M1131-200 | Biovision | each | 255.6 EUR |
mRNAExpress Tail PCR mix (5uM) |
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MR-TAIL-PR | SBI | 100 ul | 111 EUR |
Drop Delay Calibration Particles |
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DDCP-70-2 | Spherotech | 2 mL | 339.6 EUR |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Drop Delay Calibration Particles |
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DDCP-70-20 | Spherotech | 20 mL | 2301.6 EUR |
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone. |
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Taq 5x PCR Mix 12.5 mM |
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BT10402 | Bioatlas | 250rxn | 130.8 EUR |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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Drop Funnel Rotaflo SC 50ml |
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QD312 | Scientific Laboratory Supplies | PK5 | 637.2 EUR |
amfiSure Prime PCR Master Mix |
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P1311-025 | GenDepot | 5X50 rxns | 193.2 EUR |
amfiSure Prime PCR Master Mix |
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P1311-050 | GenDepot | 10X50 rxns | 262.8 EUR |
amfiSure Prime PCR Master Mix |
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P1311-125 | GenDepot | 15X50 rxns | 327.6 EUR |
amfiSure Prime PCR Master Mix |
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P1311-250 | GenDepot | 50x50 rxns | 886.8 EUR |
amfiSure Prime PCR Master Mix |
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P1311-500 | GenDepot | 100x50 rxns | 1485.6 EUR |
amfiSure Advanced PCR Master Mix |
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P2311-025 | GenDepot | 5X50 rxns | 198 EUR |
amfiSure Advanced PCR Master Mix |
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P2311-050 | GenDepot | 10X50 rxns | 267.6 EUR |
amfiSure Advanced PCR Master Mix |
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P2311-125 | GenDepot | 15X50 rxns | 338.4 EUR |
amfiSure Advanced PCR Master Mix |
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P2311-250 | GenDepot | 50x50 rxns | 902.4 EUR |
amfiSure Advanced PCR Master Mix |
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P2311-500 | GenDepot | 100x50 rxns | 1534.8 EUR |
Funnel Pyrex Cyl Drop Gr 1Ltr |
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FUN4026 | Scientific Laboratory Supplies | EACH | 249.6 EUR |
amfiSure ONE PCR Master Mix(2X) |
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P7000-005 | GenDepot | 5x1 ml | 262.8 EUR |
amfiSure ONE PCR Master Mix(2X) |
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P7000-010 | GenDepot | 10x1 ml | 380.4 EUR |
amfiSure ONE PCR Master Mix(2X) |
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P7000-050 | GenDepot | 50x1 ml | 1453.2 EUR |
amfiSure ONE PCR Master Mix(2X) |
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P7000-100 | GenDepot | 100x1 ml | 2522.4 EUR |
Drop Funnel Cyl Rota S/C 100ml |
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QD1/21GP | Scientific Laboratory Supplies | EACH | 109.2 EUR |
Drop Funnel Cyl Rota S/C 100ml |
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QD1/22GP | Scientific Laboratory Supplies | EACH | 116.4 EUR |
Drop Funnel Cyl Rota S/C 250ml |
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QD1/32GP | Scientific Laboratory Supplies | EACH | 166.8 EUR |
Drop Funnel Cylind Rota S/C 50ml |
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QD1/11GP | Scientific Laboratory Supplies | EACH | 102 EUR |
Watershed Hanging Drop Cover Slip Endstation |
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M-WS180320-24WCSES | MiTeGen | 1 UNIT | 256 EUR |
Description: Watershed Hanging Drop Cover Slip Endstation |
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Taq PCR Master Mix (2X, Red Dye) |
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BS9297 | Bio Basic | 1ml | 101.76 EUR |
Taq PCR Master Mix (2X, Red Dye) |
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BS9298 | Bio Basic | 5ml | 227.04 EUR |
Taq PCR Master Mix (2X, Blue Dye) |
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BS9295 | Bio Basic | 1ml | 101.76 EUR |
Taq PCR Master Mix (2X, Blue Dye) |
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BS9296 | Bio Basic | 5ml | 227.04 EUR |
2×Taq PCR Master Mix(with dye) |
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K1034-1 | ApexBio | 1 ml | 87.6 EUR |
2×Taq PCR Master Mix(with dye) |
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K1034-100 | ApexBio | 100×1 ml | 950.4 EUR |
2×Taq PCR Master Mix(with dye) |
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K1034-20 | ApexBio | 20×1 ml | 338.4 EUR |
2×Taq PCR Master Mix(with dye) |
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K1034-5 | ApexBio | 5×1 ml | 142.8 EUR |
2×Taq PCR Master Mix(with dye) |
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K1034-50 | ApexBio | 50×1 ml | 616.8 EUR |
Drop Funnel P/Equal R S/C 100ml |
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QDE100/22 | Scientific Laboratory Supplies | EACH | 192 EUR |
HotStart PCR Master mix (2X, Green Dye) |
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BS9291 | Bio Basic | 5X1mL, 5ml | 251.4 EUR |
HotStart PCR Master mix (2X, Green Dye) |
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BS9292 | Bio Basic | 1ml | 115.68 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-001 | GenDepot | 1ml | 336 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-002 | GenDepot | 2x1ml | 578.4 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-005 | GenDepot | 5x1ml | 993.6 EUR |
amfiSure Ultra Fidelity PCR Master Mix(2X) |
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P0346-010 | GenDepot | 10x1ml | 1632 EUR |
amfiFusion High Fidelity PCR Master Mix(2X) |
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P0342-010 | GenDepot | 2x50 rxns | 303.6 EUR |
amfiFusion High Fidelity PCR Master Mix(2X) |
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P0342-025 | GenDepot | 5X50 rxns | 546 EUR |
amfiFusion High Fidelity PCR Master Mix(2X) |
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P0342-050 | GenDepot | 10X50 rxns | 934.8 EUR |
amfiFusion High Fidelity PCR Master Mix(2X) |
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P0342-100 | GenDepot | 20x50 rxns | 1599.6 EUR |
Reverse Transcriptase RT PCR Master Mix (5X) |
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MB1002-100reactions | BosterBio | 100 reactions | 616.8 EUR |
Reverse Transcriptase RT PCR Master Mix (5X) |
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MB1002-200reactions | BosterBio | 200 reactions | 895.2 EUR |
Reverse Transcriptase RT PCR Master Mix (5X) |
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MB1002-25reactions | BosterBio | 25 reactions | 226.8 EUR |
Extra T7 gRNA PCR primer mix (5 uM) |
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CAS510A-PR | SBI | 50 assays | 99 EUR |
Taq 5x PCR Mix Ready-to-Load 12.5 mM |
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BT10502 | Bioatlas | 250rxn | 136.8 EUR |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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ReadiUseâ„¢ dNTP Mix Set *10 mM PCR Grade* |
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17258-1mL | AAT Bioquest | 1 mL | 79 EUR |
Description: 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. |
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Original INTELLI-PLATE 96-2; 2-well sitting drop, 120 plates |
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MAR-102-0011-00 | MiTeGen | 120 PLATES | 990 EUR |
Description: Original INTELLI-PLATE 96-2; 2-well sitting drop, 120 plates |
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2x Genotyping PCR Ready Master Mix (250 x 20µL rxn) |
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T403 | 101Bio | - | Ask for price |
INTELLI-PLATE 96-2 flat bottom; 2-well sitting drop, 120 plates |
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MAR-102-0001-01 | MiTeGen | 120 PLATES | 990 EUR |
Description: INTELLI-PLATE 96-2 flat bottom; 2-well sitting drop, 120 plates |
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INTELLI-PLATE 96-2 low profile; 2-well sitting drop, 120 plates |
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MAR-102-0001-10 | MiTeGen | 120 PLATES | 990 EUR |
Description: INTELLI-PLATE 96-2 low profile; 2-well sitting drop, 120 plates |
The phylogenetic analyses utilizing parsimony and Bayesian inference strategies with Chanos/Elops as outgroup respectively means that the new kind is nearer to ellimmichthyiform genus Diplomystus than to another fishes, though there are some discrepancies between the two standards and totally different outgroups used. It shares with Diplomystus the excessive supraoccipital crest, pelvic-fin insertion upfront of dorsal fin origin, and the quantity of predorsal scutes greater than 20. The new kind represents the youngest ellimmichthyiform fish report in the world. Its discovery signifies that the members of the Ellimmichthyiformes had a wider distribution vary and a longer evolutional historical past than beforehand recognized.