Skip to content

CelltaGen

셀타젠 Genetic Genotyping

Menu
  • Home
  • RNA
  • Assay
  • DNA
  • Plant
  • Guinea
  • Distributors
Menu
SMR peptide antagonizes mortalin promoted release of extracellular vesicles and affects mortalin protection from complement-dependent cytotoxicity in breast cancer cells and leukemia cells.

SMR peptide antagonizes mortalin promoted release of extracellular vesicles and affects mortalin protection from complement-dependent cytotoxicity in breast cancer cells and leukemia cells.

Posted on May 10, 2020May 10, 2020 by Frank

Background: Mortalin/GRP-75/mt-hsp70 is a mitochondrial chaperone protein, discovered in the cytoplasm, endoplasmic reticulum and cytoplasmic vesicles.

It features in many mobile processes similar to mitochondrial biogenesis, intramobile trafficking, cell proliferation, signaling, immortalization and tumorigenesis.

Thus, inhibition of mortalin is a promising avenue for cancer remedy. Previous research in our lab have recommended that mortalin contributes to breast cancer improvement and development.

We confirmed that tumor furthermobile vesicle secretion was decreased by knockdown of mortalin expression utilizing HIV-1 Nef SMR peptides. Specifically, these peptides can block furthermobile vesicle secretion and mediate cell cycle arrest in MDA-MB-231 and MCF-7 breast cancer cells. 

Aims: This research goals to research additional the operate and mechanism of interplay of PEG-SMR-CLU and SMR-CPP peptides with the chaperone protein mortalin and to discover the impact of SMR-derived peptides and mortalin expression on furthermobile vesicle release and complement dependent cell toxicity in human breast cancer and leukemia cell lines. 

Results: Our outcomes demonstrated further results reversing the tumorigenicity of these cells. First, the modified SMRwt peptides diminished the expression of the mesenchymal marker vimentin (VIM).

Second, publicity to the SMRwt peptide inhibited mortalin and complement C9 expression in MDA-MB-231, MCF-7 breast cancer cells and Okay562 leukemia cells as measured by the Western blot evaluation.

SMR peptide antagonizes mortalin promoted release of extracellular vesicles and affects mortalin protection from complement-dependent cytotoxicity in breast cancer cells and leukemia cells.
SMR peptide antagonizes mortalin promoted release of extracellular vesicles and affects mortalin protection from complement-dependent cytotoxicity in breast cancer cells and leukemia cells.

Third, the SMRwt peptides blocked the cancer cells’ capability to release furthermobile vesicles, which we noticed blocked furthermobile vesicle-mediated release of complement, re-establishing enhances mediated cell loss of life in these peptide-treated cells. 

Methods: We developed a collection of peptides derived from the Secretion Modification Region (SMR) of HIV-1 Nef protein, modified by the addition of both a cell-penetrating peptide (CPP), a positively charged arginine-rich peptide derived from HIV-1 regulatory protein Tat, or a Clusterin-binding peptide (CLU), a molecular chaperone concerned in protein secretion.

Both CPP and CLU peptide sequences had been added on the C-terminus of the Nef SMR peptide. The CLU-containing peptides had been additionally modified with polyethylene glycol (PEG) to reinforce solubility.

After therapy of cells with the peptides, we used the MTT cell viability and complement-mediated cytotoxicity assays to substantiate the inhibitory function of modified SMRwt peptides on the proliferation of MDA-MB-231 and MCF-7 breast cancer cells and Okay562 leukemia cells.

Flow cytometry was used to find out complement mediated cell apoptosis and loss of life. Western blot evaluation was used to trace SMR peptides impression on expression of mortalin, vimentin and complement C9 and to measure the expression of furthermobile vesicle proteins.

NanoSight evaluation and acetylcholinesterase (AChE) assay had been used for measuring furthermobile vesicles particle measurement and focus and acetylcholinesterase. 

Conclusions: Mortalin promotes cell proliferation, metastasis, angiogenesis, downregulate apoptotic signaling. Thus, mortalin is a possible therapeutic goal for cancer immunotherapy. The novel SMRwt peptides antagonize the features of mortalin, blocking tumor furthermobile vesicle release and furthermobile vesicle-mediated release of complement.

This results in decreases in breast cancer cell metastasis and permits normal therapy of these late stage tumor cells, thus having necessary scientific implications for late stage breast cancer chemotherapy. These findings help additional investigation into the therapeutic worth of the SMR peptide in cancer metastasis.

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Recent Posts

  • Yalongaphaenops erwini gen. et sp. nov., the world’s most high-altitude hypogean trechine beetle from China (Coleoptera, Carabidae, Trechinae)
  • Inopinatum lactosum gen. & comb. nov., the first yeast-like fungus in Leotiomycetes
  • Reappraisal of Immotthia in Dictyosporiaceae, Pleosporales: Introducing Immotthia bambusae sp. nov. and Pseudocyclothyriella clematidis comb. et gen. nov. Based on Morphology and Phylogeny
  • Harenicola maris gen. nov., sp. nov. isolated from the Sea of Japan shallow sediments
  • Paucimyces polynucleatus gen. nov, sp. nov., a novel polycentric genus of anaerobic gut fungi from the faeces of a wild blackbuck antelope

RSS Trends in Genetics

  • Earth Biogenome Project: present status and future plans
  • Mitochondrial genome engineering coming-of-age
  • Machine learning approaches to explore digenic inheritance
  • A new Down syndrome rat model races forward
  • The evolution of gene regulation on sex chromosomes
  • Retooling conditional gene expression using a floxed exon
  • The versatile relationships between eIF4E and eIF4E-interacting proteins
  • Into the multiverse: advances in single-cell multiomic profiling
  • DNA methylation: a historical perspective
  • Structure-specific nucleases: role in Okazaki fragment maturation

Categories

  • 4 Host
  • 96T
  • apoptosis
  • array
  • Assay
  • Bacteria
  • DNA
  • Establishment and characterization of HBV-associated B lymphocytes with an immortalization potential.
  • GMO
  • Goat
  • Green
  • Guinea
  • Malignant transformation in a Breast Adenomyoepithelioma Caused by Amplification of c-MYC: A Common pathway to Cancer in a Rare Entity.
  • Mechanisms underlying the activation of TERT transcription and telomerase activity in human cancer: old actors and new players.
  • Pig
  • Pigeon
  • PITX1 protein interacts with ZCCHC10 to regulate hTERT mRNA transcription.
  • Plant
  • plasmid
  • Plate
  • PTPN14 degradation by high-risk human papillomavirus E7 limits keratinocyte differentiation and contributes to HPV-mediated oncogenesis.
  • RNA
  • SMR peptide antagonizes mortalin promoted release of extracellular vesicles and affects mortalin protection from complement-dependent cytotoxicity in breast cancer cells and leukemia cells.

Recent Posts

  • Yalongaphaenops erwini gen. et sp. nov., the world’s most high-altitude hypogean trechine beetle from China (Coleoptera, Carabidae, Trechinae)
  • Inopinatum lactosum gen. & comb. nov., the first yeast-like fungus in Leotiomycetes
  • Reappraisal of Immotthia in Dictyosporiaceae, Pleosporales: Introducing Immotthia bambusae sp. nov. and Pseudocyclothyriella clematidis comb. et gen. nov. Based on Morphology and Phylogeny
  • Harenicola maris gen. nov., sp. nov. isolated from the Sea of Japan shallow sediments
  • Paucimyces polynucleatus gen. nov, sp. nov., a novel polycentric genus of anaerobic gut fungi from the faeces of a wild blackbuck antelope

RSS Trends in Genetics

  • Earth Biogenome Project: present status and future plans
  • Mitochondrial genome engineering coming-of-age
  • Machine learning approaches to explore digenic inheritance
  • A new Down syndrome rat model races forward
  • The evolution of gene regulation on sex chromosomes
  • Retooling conditional gene expression using a floxed exon
  • The versatile relationships between eIF4E and eIF4E-interacting proteins
  • Into the multiverse: advances in single-cell multiomic profiling
  • DNA methylation: a historical perspective
  • Structure-specific nucleases: role in Okazaki fragment maturation

Categories

  • 4 Host
  • 96T
  • apoptosis
  • array
  • Assay
  • Bacteria
  • DNA
  • Establishment and characterization of HBV-associated B lymphocytes with an immortalization potential.
  • GMO
  • Goat
  • Green
  • Guinea
  • Malignant transformation in a Breast Adenomyoepithelioma Caused by Amplification of c-MYC: A Common pathway to Cancer in a Rare Entity.
  • Mechanisms underlying the activation of TERT transcription and telomerase activity in human cancer: old actors and new players.
  • Pig
  • Pigeon
  • PITX1 protein interacts with ZCCHC10 to regulate hTERT mRNA transcription.
  • Plant
  • plasmid
  • Plate
  • PTPN14 degradation by high-risk human papillomavirus E7 limits keratinocyte differentiation and contributes to HPV-mediated oncogenesis.
  • RNA
  • SMR peptide antagonizes mortalin promoted release of extracellular vesicles and affects mortalin protection from complement-dependent cytotoxicity in breast cancer cells and leukemia cells.

Recent Posts

  • Yalongaphaenops erwini gen. et sp. nov., the world’s most high-altitude hypogean trechine beetle from China (Coleoptera, Carabidae, Trechinae)
  • Inopinatum lactosum gen. & comb. nov., the first yeast-like fungus in Leotiomycetes
  • Reappraisal of Immotthia in Dictyosporiaceae, Pleosporales: Introducing Immotthia bambusae sp. nov. and Pseudocyclothyriella clematidis comb. et gen. nov. Based on Morphology and Phylogeny
  • Harenicola maris gen. nov., sp. nov. isolated from the Sea of Japan shallow sediments
  • Paucimyces polynucleatus gen. nov, sp. nov., a novel polycentric genus of anaerobic gut fungi from the faeces of a wild blackbuck antelope
© 2022 CelltaGen | Powered by Superbs Personal Blog theme